A professional image and video annotation tool for researchers. Mark points, measure distances, and record angles on microscopy images. Calibrate a ruler to real physical dimensions and export data to JSON or Excel with one click.
DEMO · Annotation workbench preview
Each tool is designed to support reproducible research data — from pixel coordinates to physical dimensions, every step is traceable.
Point coordinates, distance (line segment), and angle (three points) cover common measurement needs with seamless switching.
Ctrl + drag to draw a calibration line of known length, then enter its real-world size — image coordinates convert instantly to physical units.
A magnifying loupe follows the cursor to keep edge pixels clearly visible, aiding sub-pixel positioning.
PNG, JPG, BMP images and MP4, AVI video are all handled in one interface — no conversion needed.
Export annotations to JSON or Excel with one click, or import existing data to continue working seamlessly.
Frame-by-frame control with synced annotation logging lets you quantify motion and structural change over time.
From opening an image to exporting data, one walkthrough is enough to get started.
Drag a file into the window or open from the menu — common research formats are supported.
Ctrl + drag to draw a calibration line of known length, then enter its true physical size.
Combine point, distance, and angle modes as needed, using the real-time magnifier for precise positioning.
Export annotations and measurements as JSON or Excel and bring them straight into your analysis pipeline.
Please read the license agreement below before downloading. A license confirmation dialog appears on first launch and must be accepted before use.
A standalone Windows installer with the full runtime bundled — no separate Python installation or other dependencies required.
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If you use this software in an academic publication, please cite it as follows:
By using this software, you confirm that you have read, understood, and agree to the following terms.
MolFocus — Object Annotation Tool
Copyright © 2025 Wang Tao, Institute of Chemistry, Chinese Academy of Sciences. All Rights Reserved.
This software and its documentation are the intellectual property of Wang Tao, Institute of Chemistry, Chinese Academy of Sciences. Unauthorized reproduction, distribution, or use of this software, in whole or in part, is strictly prohibited without prior written permission from the author.
This software is provided "as is," without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose, and noninfringement. In no event shall the author or copyright holder be liable for any claim, damages, or other liability, whether in an action of contract, tort, or otherwise, arising from, out of, or in connection with the software or the use or other dealings in the software.
By using this software, you acknowledge that you have read, understood, and agreed to be bound by the terms and conditions of this agreement.
Copyright © 2025 Wang Tao, Institute of Chemistry, Chinese Academy of Sciences. All Rights Reserved.
This software and all its intellectual property rights belong to Wang Tao (Institute of Chemistry, Chinese Academy of Sciences). No individual or organization may reproduce, distribute, modify, or use all or part of this software in any form without prior written permission from the author.
This software is provided "as is," without any express or implied warranties, including but not limited to the warranties of merchantability, fitness for a particular purpose, and noninfringement. In no event shall the author or copyright holder be liable for any claim, damages, or other liability arising from contract, tort, or otherwise in connection with the software or its use.
By using this software, you confirm that you have read, understood, and agree to be bound by the terms and conditions of this agreement.